AP Biology No. 8: Bacterial Transformation Kit
Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3. Aligned to the Next Generation Science Standards (NGSS)*
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Students will genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. They will then examine the number of resistant bacteria to determine the efficiency of the transformation procedure. Teachers manual and Student Study Guide copymasters are included. Materials provided for 8 lab groups. Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3.
Aligned to the Next Generation Science Standards (NGSS)*
Kit Includes:
- 30mL Sterile Calcium Chloride 0.05M
- 16 Sterile Volumetric Pipet
- 8 Pipet Bulb
- 8 Micropipet w/Plunger 10ul
- 1 Plastic Centrifuge Tube
- 16 Microcentrifuge Tube, 1.5mL
- 8 Sterile Inoculating Loop
- 40 Petri Dish
- 4 Luria Agar, 200mL
- 1 E. Coli Culture (freeze dried)
- 1 Luria Broth 9mL Tube
Kit Contains coupon for perishable materials. Redeem by fax, phone or e-mail.
Materials sent upon redemption of coupon:
- 2 btls 0.02g Ampicillin 1000 ng
- 1 pUC19 Plasmid
- 1 Tryptic Soy Broth Tube 9mL
- 1 Tryptic Soy Agar Slants 6mL
- 1 Pipet, Sterile, Disposable
- 1 pkg Cotton Swabs, Sterile
Materials Needed but not Supplied:
- Large beaker Hot plate or waterbath
- Microwave (not necessary if melting agar in a waterbath) Markers
- Microfuge tube racks Container of ice
Recommended: Incubator (37°C)
Regulatory Note: Non Regulated
*”Next Generation Science Standards” is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.